Research Articles

Tumbic, G.W.; Li, J.; Jiang, T.; Hossan, M.Y.; Feng, C.; Thielges, M.C. Interdomain Interactions Modulate the Active Site Dynamics of Human Inducible Nitric Oxide Synthase The Journal of Physical Chemistry B, 2022, 126 (36), 6811-6819. DOI:10.1021/acs.jpcb.2c04091

Mammoser, C.C.; Agh, R.E.; Garcia, N.M.; Wang, Y.; Thielges, M.C. Altered Coordination in a Blue Copper Protein upon Association with Redox Partner Revealed by Carbon-Deuterium Vibrational Probes Physical Chemistry Chemical Physics, 2022, 24, 21588-21592. DOI: 10.1039/D2CP03314C

Mammoser, C.C.; Ramos, S.; Thielges, M.C. Active Site Hydrogen Bonding Induced in Cytochrome P450cam by Effector Putidaredoxin Biochemistry, 2021, 60 (21), 1699-1707. DOI: 10.1021/acs.biochem.1c00075

Ramos, S.; Mammoser, C.C.; Thibodeau, K.E.; Thielges, M.C. Dynamics Underlying Hydroxylation Selectivity of Cytochrome P450cam Biophysical Journal, 2021, 120 (5), 912-923. DOI: 10.1016/j.bpj.2021.01.027

Bukowski, G.S.; Horness, R.E.; Thielges, M.C. Involvement of Local, Rapid Conformational Dynamics in Binding of Flexible Recognition Motifs Journal of Physical Chemistry B 2019, 123 (40), 8387-8396. DOI: 10.1021/acs.jpcb.9b07036

Ramos, S.; Le Sueur, A.L.; Horness, R.E.; Specker, J.T.; Collins, J.A.; Thibodeau, K.E.; Thielges, M.C. Heterogeneous and Highly Dynamic Interface in Plastocyanin–Cytochrome f Complex Revealed by Site-Specific 2D-IR Spectroscopy The Journal of Physical Chemistry B2019123 (9), 2114-2122.DOI: 10.1021/acs.jpcb.8b12157

Ramos, S.; Horness, R.E.H.; Collins, J.A.; Haak, D.; Thielges, M.C. Site-Specific 2D IR Spectroscopy: A General Approach for the Characterization of Protein Dynamics with High Spatial and Temporal Resolution Physical Chemistry Chemical Physics201821, 780-788DOI: 10.1039/C8CP06146G

Bukowski, G.S.; Thielges, M.C. Residue-Specific Conformational Heterogeneity of Proline-Rich Sequences Uncovered via Infrared Spectroscopy Analytical Chemistry201890 (24), 14355-14362. DOI: 10.1021/acs.analchem.8b03813

Ramos, S.; Basom, E.J.; and Thielges, M.C. Conformational Change Induced by Putidaredoxin Binding to CO Complex of Cytochrome P450cam Characterized by 2D IR Spectroscopy. Frontiers in Molecular Biosciences2018DOI: 10.3389/fmolb.2018.00094

Basom, E.J.; Manifold, B.A.; and Thielges, M.C. Conformational Heterogeneity and the Affinity of Substrate Molecular Recognition by Cytochrome P450cam. Biochemistry201756 (25), 3248-3256. DOI: 10.1021/acs.biochem.7b00238

Le Sueur, A.L.; Ramos, S.; Ellefsen, J.D.; Cook, S.; Thielges, M.C. Evaluation of p-(13C,15N-Cyano)phenylalanine as an Extended Time Scale 2D IR Probe of Proteins. Analytical Chemistry 201789 (10), 5254-5260. DOI: 10.1021/acs.analchem.6b04650

Ramos, S.; Scott, K.J.; Horness, R.E.; Le Sueur, A.L.; and Thielges, M.C. Extended timescale 2D IR probes of proteins: p-cyanoselenophenylalanine. Physical Chemistry Chemical Physics 201719, 10081-10086. DOI: 10.1039/C7CP00403F

Basom, E.J.; Maj, M.; Cho, M.; and Thielges, M.C. Site-specific Characterization of Cytochrome P450cam Conformations by Infrared Spectroscopy. Analytical Chemistry 2016, 88, 6598-6606. DOI: 10.1021/acs.analchem.6b01520

Le Sueur, A.L.; Schaugaard, R.N.; Baik, M.-H.; and Thielges, M.C. Methionine Ligand Interaction in a Blue Copper Protein Characterized by Site-Selective Infrared Spectroscopy. Journal of the American Chemical Society 2016138, 7187-7193. DOI: 10.1021/jacs.6b03916

Horness, R.E.; Basom, E.J.; Mayer, J.P.; and Thielges, M.C. Resolution of Site-Specific Conformational Heterogeneity in Proline-Rich Molecular Recognition by Src Homology 3 Domains. Journal of the American Chemical Society 2016138 (4), 1130-1133. DOI: 10.1021/jacs.5b11999

Jordan, P.C.; Patterson, D.P.; Saboda, K.N.; Edwards, E.J.; Miettinen, H.M.; Basu, G.; Thielges, M.C.; and Douglas, T. Self-assembling Biomolecular Catalysts for Hydrogen Production. Nature Chemistry 20158, 179-185. DOI: 10.1038/nchem.2416

Le Sueur, A.L.; Horness, R.E.; and Thielges, M.C. Applications of Two-Dimensional Infrared Spectroscopy. Analyst 2015140, 4336-4349. DOI: 10.1039/C5AN00558B

Basom, E.J.; Spearman, J.W.; and Thielges, M.C. Conformational Landscape and the Selectivity of Cytochrome P450cam. Journal of Physical Chemistry B 2015119 (22), 6620-6627. DOI: 10.1021/acs.jpcb.5b03896

Horness, R.E.; Basom, E.J.; and Thielges, M.C. Site-selective characterization of Src homology 3 domain molecular recognition with cyanophenylalanine infrared probes. Analytical Methods 2015, 7, 7234-7241. DOI: 10.1039/C5AY00523J


Thielges, M.C. Transparent window 2D IR spectroscopy of proteins The Journal of Chemical Physics, 2021, 155, 040903. DOI: 10.1063/5.0052628

Tumbic, G.W; Hossan, M.Y.; Thielges, M.C. Protein Dynamics by Two-Dimensional Infrared Spectroscopy Annual Review of Analytical Chemistry, 202114, 299-321. DOI: 10.1146/annurev-anchem-091520-091009

Ramos, S.; Thielges, M.C. Site-Specific 1D and 2D IR Spectroscopy to Characterize the Conformations and Dynamics of Protein Molecular Recognition The Journal of Physical Chemistry B2019123 (17), 3551-3566.DOI: 10.1021/acs.jpcb.9b00969